• Product NameTCRZ (Phospho-Tyr142) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of TCRZ only when phosphorylated at tyrosine 142.
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 142 (G-L-Y(p)-Q-G) derived from Human TCRZ.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    T3Z; CD3-zeta; TCRZ; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD_antigen: CD247;CD247;CD3Z
• Accession No. Swiss-Prot#:P20963
  NCBI Gene ID:919
  NCBI mRNA#:NM_000734.3.
  NCBI Protein#:NP_000725.1.
• UniprotP20963
• Gene ID919;
• Calculated MW 20
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

• Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting