• Product NameHistamine H1 Receptor (Phospho-Ser398) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of Histamine H1 Receptor only when phosphorylated at serine 398.
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 398(S-H-S(p)-R-Q) derived from Human Histamine H1 Receptor .
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    H1-R;HRH1;histamine H1 receptor
• Accession No. Swiss-Prot#:P35367
  NCBI Gene ID:3269
  NCBI mRNA#:NM_000861.3.
  NCBI Protein#:NP_000852.1.
• UniprotP35367
• Gene ID3269;
• Calculated MW 55
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

• Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting