• Product NameMUC1 (Phospho-Tyr1229) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of MUC1 only when phosphorylated at tyrosine 1229.
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human MUC1.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    PUM; EMA;H23AG; MUC-1; PEM;Mucin-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;Polymorphic epithelial mucin;Tumor-associated epithelial membrane antigen;Tumor-associated mucin;CD_antigen: CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;CD227
• Accession No. Swiss-Prot#:P15941
  NCBI Gene ID:4582
  NCBI mRNA#:NM_001018016.2.
  NCBI Protein#:NP_001018016.1.
• UniprotP15941
• Gene ID4582;
• Calculated MW 170
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

• Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting