• Product NameSmad3 (Phospho-Ser423/425) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu, Ms
• Immunogen Descriptionpeptide
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    75SirT1 antibody
  hSIR2 antibody
  hSIRT1 antibody
  HST2, S. cerevisiae, homolog of antibody
  NAD dependent deacetylase sirtuin 1 antibody
  NAD dependent protein deacetylase sirtuin 1 antibody
  OTTHUMP00000198111 antibody
  OTTHUMP00000198112 antibody
  Regulatory protein SIR2 homolog 1 antibody
  SIR1_HUMAN antibody
  SIR2 antibody
  SIR2 like 1 antibody
  SIR2 like protein 1 antibody
  SIR2, S.cerevisiae, homolog-like 1 antibody
  SIR2-like protein 1 antibody
  SIR2ALPHA antibody
  SIR2L1 antibody
  Sirt1 antibody
  SirtT1 75 kDa fragment antibody
  Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody
  Sirtuin 1 antibody
  Sirtuin type 1 antibody
  
• Accession No. Swiss-Prot#:Q96EB6
  
• UniprotQ96EB6
• Gene ID23411;
• Calculated MW 110
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting