• Product NameMEK1(Phospho-S218/S222) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    I kappa B alpha antibody
  I-kappa-B-alpha antibody
  IkappaBalpha antibody
  IkB-alpha antibody
  IKBA antibody
  IKBA_HUMAN antibody
  IKBalpha antibody
  MAD 3 antibody
  MAD3 antibody
  Major histocompatibility complex enhancer-binding protein MAD3 antibody
  NF kappa B inhibitor alpha antibody
  NF-kappa-B inhibitor alpha antibody
  NFKBI antibody
  NFKBIA antibody
  Nuclear factor of kappa light chain gene enhancer in B cells antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
  
• Accession No. Swiss-Prot#:P25963
  
• UniprotP25963
• Gene ID4792;
• Calculated MW 34
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting