• Product NameSTAT1(Phospho-S727) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Ser78 of human Hsp27.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Heat shock 27kDa protein antibody
  28 kDa heat shock protein antibody
  CMT2F antibody
  DKFZp586P1322 antibody
  epididymis secretory protein Li 102 antibody
  Estrogen regulated 24 kDa protein antibody
  Estrogen-regulated 24 kDa protein antibody
  Heat shock 25kDa protein 1 antibody
  Heat shock 27 kDa protein antibody
  Heat shock 27kD protein 1 antibody
  Heat shock 27kDa protein 1 antibody
  Heat shock 28kDa protein 1 antibody
  Heat Shock Protein 27 antibody
  Heat shock protein beta 1 antibody
  Heat shock protein beta-1 antibody
  heat shock protein family B (small) member 1 antibody
  HEL-S-102 antibody
  HMN2B antibody
  HS.76067 antibody
  Hsp 25 antibody
  HSP 27 antibody
  Hsp 28 antibody
  Hsp B1 antibody
  Hsp25 antibody
  HSP27 antibody
  Hsp28 antibody
  HspB1 antibody
  HSPB1_HUMAN antibody
  SRP27 antibody
  Stress responsive protein 27 antibody
  Stress-responsive protein 27 antibody
  
• Accession No. Swiss-Prot#:P04792
  
• UniprotP04792
• Gene ID3315;
• Calculated MW 27
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting