• Product NameMLKL (Phospho-S345) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.JM92-37
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    9130019I15Rik antibody
  FLJ34389 antibody
  hMLKL antibody
  Mixed lineage kinase domain like antibody
  Mixed lineage kinase domain like protein antibody
  Mixed lineage kinase domain like pseudokinase antibody
  Mixed lineage kinase domain-like protein antibody
  Mlkl antibody
  MLKL_HUMAN antibody
  
• Accession No. Swiss-Prot#:Q9D2Y4
• UniprotP00918
• Gene ID760;
• Calculated MW 54 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting