• Product NameLC3A Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total LC3A protein.
• Immunogen DescriptionPeptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Microtubule-associated protein 1 light chain 3 alpha;MAP1A/1B light chain 3 A ;MAP1A/MAP1B LC3 A ;MAP1 light chain 3-like protein 1 ;Autophagy-related protein LC3 A
• Accession No. Swiss-Prot#:Q9H492
  NCBI Gene ID:84557
  NCBI mRNA#:NM_032514.2
  NCBI Protein#:NP_115903.1
• UniprotQ9H492
• Gene ID84557;
• Calculated MW 18 16
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

• Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Probably involved in formation of autophagosomal vacuoles (autophagosomes).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting