• Product NameHDAC4/HDAC5/HDAC9 (Ab-246/259/220) Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total HDAC4/HDAC5/HDAC9 protein.
• Immunogen DescriptionPeptide sequence around aa.244~248/257~261/218~222 (T-A-S-E-P) derived from Human HDAC4/HDAC5/HDAC9.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    HD4/HD5/HD9
• Accession No. Swiss-Prot#:P56524 Q9UQL6 Q9UKV0
  NCBI Gene ID:9759 10014 9734
  NCBI mRNA#:NM_006037.3 NM_001015053.1 NM_014707.1
  NCBI Protein#:NP_006028.2 NP_001015053.1 NP_055522.1
• UniprotP56524
• Gene ID9759;
• Calculated MW 140 124 111
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

• Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting