• Product NameAMPKα1 (Phospho-Ser496)Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of AMPKα1 only when phosphorylated at serine 496.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 496 (S-G- S(P)-V-S) derived from Human AMPKα1.
• Target NameAMPKa1
• ModificationPhospho
• Alternative Names    AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1
• Accession No. Swiss-Prot: Q13131
  NCBI Protein: NP_006242.5
• UniprotQ13131
• Gene ID5562;
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. IHC 1:100 - 1:300.  IF 1:50-200

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser496)Antibody #11174 (left) or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1(Phospho-Ser496)Antibody #11174.
• Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser496)Antibody #11174.
• Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKα1 (Phospho-Ser496) Antibody #11174.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting