• Product NameRAB3D Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RAB3D protein.
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human RAB3D, member RAS oncogene family
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    GOV, D2-2, RAB16, RAD3D
• Accession No. Swiss-Prot#:O95716
  NCBI Gene ID:9545
  NCBI Protein#:NP_004274
• UniprotO95716
• Gene ID9545;
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

The RAB3D cDNA encodes a 24-kD protein as determined by in vitro translation. The deduced 219-amino acid sequence shows highest homology to the RAB3 subfamily of low molecular mass GTP-binding proteins. Northern blot analysis of various human hematopoietic cell lines showed a single transcript of 4.1 kb present at variable levels in each cell line, with highest expression in granulocytic lines. RAB3D was also highly expressed in YJ and HL-60 cells in response to retinoic acid treatment.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting