• Product NamePOU2AF1 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total POU2AF1 protein.
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human POU class 2 associating factor 1
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    BOB1, OBF1, OCAB, OBF-1
• Accession No. Swiss-Prot#:Q16633
  NCBI Gene ID:5450
  NCBI Protein#:NP_006226
• UniprotQ16633
• Gene ID5450;
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene. POU2AF1 has been shown to interact with POU2F1 and SIAH1. POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene.Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It encourages the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is necessary for the response of B-cells to antigens and required for the formation of germinal centers.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting