• Product Namephospholipase C eta 1 isoform b antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 530 and 823 of Human PLCH1
• Target Namephospholipase C eta 1 isoform b
• Accession No. Swiss-Prot:Q4KWH8
  Gene ID:23007
• UniprotQ4KWH8
• Gene ID23007;
• Concentration 0.9mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 185kd
  Western blotting: 1:500-1:3000
  

PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: A431
  B: Hep G2
  5% SDS PAGE
  Primary antibody diluted at 1: 500

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting