• Product NameCytokeratin 7 -Cytoskeleton Marker Conjugated Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• Species ReactivityHu
• Immunogen DescriptionThis antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C -terminal cytokeratin 7.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    CK 7 antibody
  CK-7 antibody
  CK7 antibody
  Cytokeratin 7 antibody
  Cytokeratin-7 antibody
  D15Wsu77e antibody
  K2C7 antibody
  K2C7_HUMAN antibody
  K7 antibody
  Keratin 7 antibody
  Keratin 7, type II antibody
  Keratin type II cytoskeletal 7 antibody
  Keratin, 55K type II cytoskeletal antibody
  Keratin, simple epithelial antibody
  Keratin, simple epithelial type I, K7 antibody
  Keratin, type II cytoskeletal 7 antibody
  Keratin-7 antibody
  Krt2-7 antibody
  KRT7 antibody
  MGC11625 antibody
  MGC129731 antibody
  MGC3625 antibody
  Sarcolectin antibody
  SCL antibody
  Type II mesothelial keratin K7 antibody
  Type-II keratin Kb7 antibody
  
• Accession No. Swiss-Prot#:P08729
  
• UniprotP08729
• Gene ID3855;
• Calculated MW 51 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting