• Product NameALAS2 Conjugated Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionpeptide
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    5 @aminolevulinate synthase erythroid specific antibody
  5 aminolevulinate synthase 2 antibody
  5 aminolevulinate synthase 5 aminolevulinate synthase 2 antibody
  5 aminolevulinate synthase erythroid specific mitochondrial antibody
  5 aminolevulinic acid synthase 2 antibody
  5 aminolevulinic acid synthase antibody
  5-aminolevulinate synthase antibody
  5-aminolevulinic acid synthase 2 antibody
  Alas 2 antibody
  ALAS antibody
  ALAS E antibody
  ALAS, erythroid antibody
  ALAS-E antibody
  Alas2 antibody
  ALASE antibody
  Aminolevulinate delta synthase 2 antibody
  Aminolevulinic acid synthase 2, erythroid antibody
  ANH1 antibody
  ASB antibody
  Delta ALA synthase 2 antibody
  Delta ALA synthetase antibody
  Delta aminolevulinate synthase 2 antibody
  Delta aminolevulinate synthase antibody
  Delta-ALA synthase 2 antibody
  Delta-aminolevulinate synthase 2 antibody
  Erythroid specific ALAS antibody
  erythroid-specific antibody
  FLJ93603 antibody
  HEM0_HUMAN antibody
  mitochondrial antibody
  OTTHUMP00000023388 antibody
  OTTHUMP00000023389 antibody
  OTTMUSP00000020679 antibody
  RP23-338A17.1 antibody
  SIDBA1 antibody
  XLDPP antibody
  XLEPP antibody
  XLSA antibody
  
• Accession No. Swiss-Prot#:P22557
  
• UniprotP22557
• Gene ID212;
• Calculated MW 60 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting