• Product NamePirh2 Conjugated Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Species ReactivityHu, Rt
• Immunogen DescriptionRecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Androgen receptor N terminal interacting protein antibody
  Androgen receptor N-terminal-interacting protein antibody
  ARNIP antibody
  CH-rich-interacting match with PLAG1 antibody
  CHIMP antibody
  E3 ubiquitin-protein ligase Pirh2 antibody
  hARNIP antibody
  hPirh2 antibody
  p53 induced protein with a RING H2 domain antibody
  p53-induced RING-H2 protein antibody
  PIRH2E antibody
  PIRH2F antibody
  PRO1996 antibody
  RCHY1 antibody
  Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase antibody
  RING finger and CHY zinc finger domain-containing protein 1 antibody
  RING finger protein 199 antibody
  RNF199 antibody
  ZCHY antibody
  ZFP 363 antibody
  zinc finger CHY type antibody
  Zinc finger protein 363 antibody
  ZN363_HUMAN antibody
  ZNF363 antibody
  
• Accession No. Swiss-Prot#:Q96PM5
  
• UniprotQ96PM5
• Gene ID25898;
• Calculated MW 30 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Pirh2, also known as Androgen receptor N-terminal-interacting protein (ARNIP), ZN363 or CHIMP, has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. The protein physically interacts with p53 and the resulting degradation of p53 renders Pirh2 an oncogenic protein as the loss of p53 function contributes to malignant tumor development. The gene encoding for the protein maps to chromosome 4q21.1 and transcription of this gene is regulated by p53. Pirh2 expression decreases the level of p53 and a decrease of endogenous Pirh2 expression ups p53 levels. Pirh2 is therefore considered, together with MDM2, to be acting as a negative regulator of p53 function.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting