• Product NamePINCH Conjugated Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    2310016J22Rik antibody
  4921524A02Rik antibody
  AI507642 antibody
  AU021743 antibody
  AW551584 antibody
  C430041B13Rik antibody
  LIM and senescent cell antigen like domains 1 antibody
  LIM and senescent cell antigen-like-containing domain protein 1 antibody
  LIM zinc finger domain containing 1 antibody
  LIM-type zinc finger domains 1 antibody
  Lims1 antibody
  LIMS1_HUMAN antibody
  Lims1l antibody
  Particularly interesting new Cys His protein 1 antibody
  Particularly interesting new Cys His protein antibody
  Particularly interesting new Cys-His protein 1 antibody
  PINCH 1 antibody
  PINCH antibody
  PINCH-1 antibody
  PINCH1 antibody
  Renal carcinoma antigen NY REN 48 antibody
  Renal carcinoma antigen NY-REN-48 antibody
  senescent cell antigen antibody
  wu:fc32c03 antibody
  zgc:112533 antibody
  
• Accession No. Swiss-Prot#:P48059
  
• UniprotP48059
• Gene ID3987;
• Calculated MW 37 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting