• Product NameSmad4 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    (Small) Mothers Against Decapentaplegic antibody
  Deleted in Pancreatic Carcinoma 4 antibody
  Deleted in Pancreatic Carcinoma antibody
  Deleted in pancreatic carcinoma locus 4 antibody
  Deletion target in pancreatic carcinoma 4 antibody
  DPC 4 antibody
  DPC4 antibody
  hSMAD4 antibody
  JIP antibody
  MAD homolog 4 antibody
  MAD mothers against decapentaplegic Drosophila homolog 4 antibody
  MAD mothers against decapentaplegic homolog 4 antibody
  MADH 4 antibody
  MADH4 antibody
  Med antibody
  Medea antibody
  Mothers against decapentaplegic homolog 4 antibody
  Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody
  Mothers against DPP homolog 4 antibody
  MYHRS antibody
  OTTHUMP00000163548 antibody
  SMA- and MAD-related protein 4 antibody
  SMAD 4 antibody
  SMAD family member 4 antibody
  SMAD mothers against DPP homolog 4 antibody
  SMAD4 antibody
  SMAD4_HUMAN antibody
  
• Accession No. Swiss-Prot#:Q13485
  
• UniprotQ13485
• Gene ID4089;
• Calculated MW 60 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting