• Product NameAKT1/2/3 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    AKT antibody
  AKT1 antibody
  AKT1 kinase antibody
  AKT1m antibody
  AKT2 antibody
  AKT2 kinase antibody
  Akt3 antibody
  AKT3_HUMAN antibody
  CAKT antibody
  CWS6 antibody
  DKFZp434N0250 antibody
  HIHGHH antibody
  kinase Akt1 antibody
  MGC99656 antibody
  MPPH antibody
  Murine thymoma viral (v-akt) homolog 2 antibody
  PKB ALPHA antibody
  PKB antibody
  PKB beta antibody
  PKB gamma antibody
  PKB-GAMMA antibody
  PKB/Akt antibody
  PKBALPHA antibody
  PKBB antibody
  PKBBETA antibody
  PKBG antibody
  PKBGAMMA antibody
  PRKBA antibody
  PRKBB antibody
  PRKBG antibody
  Protein kinase Akt 2 antibody
  Protein kinase Akt-3 antibody
  Protein kinase B alpha antibody
  Protein kinase B antibody
  Protein kinase B beta antibody
  Protein kinase B gamma antibody
  Proto oncogene c Akt antibody
  RAC ALPHA antibody
  RAC alpha serine/threonine protein kinase antibody
  RAC antibody
  RAC BETA antibody
  RAC beta serine/threonine protein kinase antibody
  RAC PK alpha antibody
  RAC PK beta antibody
  rac protein kinase alpha antibody
  rac protein kinase beta antibody
  RAC-gamma antibody
  RAC-gamma serine/threonine-protein kinase antibody
  RAC-PK-gamma antibody
  RACALPHA antibody
  RACalpha serine/threonine kinase antibody
  RACBETA antibody
  RACgamma antibody
  RACgamma serine/threonine protein kinase antibody
  RACPKgamma antibody
  serine threonine protein kinase antibody
  STK-2 antibody
  STK2 antibody
  thymoma viral proto oncogene 1 antibody
  thymoma viral proto oncogene antibody
  V akt murine thymoma viral oncogene homolog 1 antibody
  V akt murine thymoma viral oncogene homolog 2 antibody
  V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
  V akt murine thymoma viral oncogene homolog 3 antibody
  vakt murine thymoma viral oncogene homolog 1 antibody
  vakt murine thymoma viral oncogene homolog 2 antibody
  vakt murine thymoma viral oncogene homolog 3 antibody
  
• Accession No. Swiss-Prot#:P31749
  
• UniprotP31749
• Gene ID207;
• Calculated MW 56 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting