• Product NameADD1(Phospho-Ser726) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of ADD1only when phosphorylated at serine 726.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
• Target NameADD1
• ModificationPhospho
• Alternative Names    ADDA; Erythrocyte adducin alpha subunit;
• Accession No. Swiss-Prot: P35611
  NCBI Protein: NP_001110.2
• UniprotP35611
• Gene ID118;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 130kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12
Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51
Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70

• Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody #11182 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody #11182(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody #11182.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting