• Product NameCD99 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    12E7 antibody
  Antigen identified by monoclonal 12E7, Y homolog antibody
  Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
  CD99 antibody
  CD99 antigen antibody
  CD99 molecule antibody
  CD99_HUMAN antibody
  Cell surface antigen 12E7 antibody
  Cell surface antigen HBA 71 antibody
  Cell surface antigen O13 antibody
  E2 antigen antibody
  HBA71 antibody
  MIC 2X antibody
  MIC 2Y antibody
  MIC2 (monoclonal antibody
  12E7) antibody
  MIC2 antibody
  MIC2X antibody
  MIC2Y antibody
  MSK5X antibody
  Protein MIC2 antibody
  Surface antigen MIC2 antibody
  T cell surface glycoprotein E2 antibody
  T-cell surface glycoprotein E2 antibody
  
• Accession No. Swiss-Prot#:P14209
  
• UniprotP14209
• Gene ID4267;
• Calculated MW 25 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting