• Product NameCD32 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    CD32 antibody
  CD32A antibody
  CD32B antibody
  CD32C antibody
  CDw32 antibody
  Fc fragment of IgG low affinity IIa receptor antibody
  Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
  Fc fragment of IgG low affinity IIb receptor antibody
  Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
  Fc fragment of IgG low affinity IIc receptor antibody
  Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
  Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
  Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
  Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
  Fc gamma receptor IIB antibody
  Fc gamma receptor IIC antibody
  Fc gamma RII a antibody
  Fc gamma RII b antibody
  Fc gamma RII c antibody
  Fc gamma RIIb antibody
  Fc gamma RIIc antibody
  Fc receptor, IgG, low affinity IIb antibody
  Fc-gamma RII-a antibody
  Fc-gamma RII-b antibody
  Fc-gamma RII-c antibody
  Fc-gamma-RIIa antibody
  FC-gamma-RIIB antibody
  Fc-GAMMA-RIIC antibody
  Fc[g]RII antibody
  FCG2 antibody
  FCG2A_HUMAN antibody
  FcGR antibody
  FCGR2 antibody
  FCGR2A antibody
  FCGR2A1 antibody
  FCGR2B antibody
  FCGR2C antibody
  Fcgr3 antibody
  Fcgr3a antibody
  FcgRII antibody
  Fcr-2 antibody
  Fcr-3 antibody
  FcRII a antibody
  FCRII antibody
  FcRII b antibody
  FcRII c antibody
  FcRII-a antibody
  FcRIIC antibody
  IGFR2 antibody
  IgG Fc receptor II a antibody
  IgG Fc receptor II b antibody
  IgG Fc receptor II beta antibody
  IgG Fc receptor II c antibody
  IgG Fc receptor II-a antibody
  IgG Fc receptor II-b antibody
  IgG Fc receptor II-c antibody
  Immunoglobulin G Fc receptor II antibody
  Immunoglobulin G Fc receptor II c antibody
  Low affinity immunoglobulin gamma Fc region receptor II antibody
  Low affinity immunoglobulin gamma Fc region receptor II-a antibody
  Low affinity immunoglobulin gamma Fc region receptor II-b antibody
  Low affinity immunoglobulin gamma Fc region receptor II-c antibody
  Ly-17 antibody
  Ly-m20 antibody
  LyM-1 antibody
  Lymphocyte antigen 17 antibody
  
• Accession No. Swiss-Prot#:P12318
  
• UniprotP12318
• Gene ID2212;
• Calculated MW 45 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG (1,2). CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells (1-4). IgG can noncooperatively bind either one or two highly glycosylated CD32 molecules, and this binding delivers a negative signal for B cells (1,2,5). CD32 exists as several isoforms that are produced by alternative splicing of three distinct genes, A, B, and C (2,6). These isoforms are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC (1,2,6). All isoforms are present on monocytes, placental trophoblasts and endothelial cells (1,6). In addition, the FcgRIIB forms are present on B lymphocytes, and the FcgRIIA and FcgRIIC forms are found on neutrophils (1,6).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting