• Product NameRPE65 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    All-trans-retinyl-palmitate hydrolase antibody
  LCA 2 antibody
  LCA2 antibody
  Leber congenital amaurosis antibody
  mRPE 65 antibody
  mRPE65 antibody
  p63 antibody
  rd 12 antibody
  rd12 antibody
  Retinal pigment epithelium specific 61 kDa protein antibody
  Retinal pigment epithelium specific 65 kDa protein antibody
  Retinal pigment epithelium specific protein antibody
  Retinal pigment epithelium specific protein 65kDa antibody
  Retinal pigment epithelium-specific 65 kDa protein antibody
  Retinitis pigmentosa 20 antibody
  Retinoid isomerohydrolase antibody
  Retinol isomerase antibody
  RP 20 antibody
  RP20 antibody
  RPE 65 antibody
  RPE65 antibody
  RPE65_HUMAN antibody
  sRPE 65 antibody
  sRPE65 antibody
  
• Accession No. Swiss-Prot#:Q16518
  
• UniprotQ16518
• Gene ID6121;
• Calculated MW 61 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65kDa protein belonging to the beta-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting