• Product NameMGEA5 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms
• Immunogen DescriptionRecombinant protein of N-terminal human MGEA5.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Beta-hexosaminidase antibody
  Beta-N-acetylglucosaminidase antibody
  Beta-N-acetylhexosaminidase antibody
  Bifunctional protein NCOAT antibody
  HEXC antibody
  Hyaluronidase in meningioma antibody
  KIAA0679 antibody
  MEA5 antibody
  Meningioma expressed antigen 5 antibody
  Meningioma-expressed antigen 5 antibody
  MGEA 5 antibody
  Mgea5 antibody
  N-acetyl-beta-D-glucosaminidase antibody
  N-acetyl-beta-glucosaminidase antibody
  NCOAT antibody
  Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
  Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
  OGA antibody
  OGA_HUMAN antibody
  Protein O-GlcNAcase antibody
  
• Accession No. Swiss-Prot#:O60502
  
• UniprotO60502
• Gene ID10724;
• Calculated MW 103 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting