Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF555 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Programmed death-1 (PD-1, CD279), a negative costimulatory molecule, is a type I glycoprotein and is inducibly expressed on T cells, B cells, natural killer T cells and activated monocytes. It is a 50-55 kDa type I transmembrane receptor, belonging to member of IgG superfamily. The intracellular domain of PD-1 contains two tyrosine-based signaling motifs (ITIM and ITSM), which can recruit the phosphatases SHP-1 and SHP-2. PD-1 can also be found on thymus and bone marrow during the development. PD-1 plays a negative regulatory role when combining with its ligands named PD-L1/PD-L2 during immune response.