• Product NameNEDD4 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC
• Species ReactivityHuman
• Immunogen DescriptionRecombinant fusion protein of human NEDD4 (NP_940682.2).
• Alternative Names    NEDD4; NEDD4-1; RPF1; E3 ubiquitin-protein ligase NEDD4
• Accession No. Swiss-Prot#:P46934
  NCBI Gene ID:4734
• UniprotP46934
• Gene ID4734;
• Calculated MW 120kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.12.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

• Immunohistochemistry of paraffin-embedded human prostate using NEDD4 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human gastric using NEDD4 at dilution of 1:200 (40x lens).
• Western blot analysis of extracts of various cell lines, using NEDD4 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting