• Product NameHO-1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human HO-1 (NP_002124.1).
• Alternative Names    HMOX1; HMOX1D; HO-1; HSP32; bK286B10; heme oxygenase 1
• Accession No. Swiss-Prot#:P09601
  NCBI Gene ID:3162
• UniprotP09601
• Gene ID3162;
• Calculated MW 32kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

• Immunohistochemistry of paraffin-embedded rat lung using HO-1 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver using HO-1 at dilution of 1:200 (40x lens).
• Western blot analysis of extracts of various cell lines, using HO-1 at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using HO-1 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting