• Product NameGRP78 / BiP Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human BiP/GRP78 (NP_005338.1).
• Alternative Names    HSPA5;BIP;GRP78;HEL-S-89n;MIF2
• Accession No. Uniprot:P11021
  GeneID:3309
• UniprotP11021
• Gene ID3309
• Calculated MW 78kDa
• SDS-PAGE MW 80KDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

• Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody.
• Immunohistochemistry of paraffin-embedded rat brain using BiP/GRP78 antibody.
• Immunohistochemistry of paraffin-embedded mouse brain using BiP/GRP78 antibody.
• Immunofluorescence analysis of PC-12 cells using BiP/GRP78 Rabbit pAb.
• Immunofluorescence analysis of U2OS cells using BiP/GRP78 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded human placenta using BiP/GRP78 antibody.
• Immunofluorescence analysis of NIH/3T3 cells using BiP/GRP78 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded human liver cancer using BiP/GRP78 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting