• Product NameCRKL Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human CRKL (NP_005198.1).
• Alternative Names    CRKL; crk-like protein
• Accession No. Swiss-Prot#:P46109
  NCBI Gene ID:1399
• UniprotP46109
• Gene ID1399;
• Calculated MW 37kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

• Western blot analysis of extracts from normal (control) and CRKL knockout (KO) HeLa cells, using CRKL at 1:3000 dilution.
• Western blot analysis of extracts of various cell lines, using CRKL at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting