• Product NameFXN / Frataxin Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human FXN / Frataxin (NP_000135.2).
• Alternative Names    FXN; CyaY; FA; FARR; FRDA; X25; frataxin
• Accession No. Swiss-Prot#:Q16595
  NCBI Gene ID:2395
• UniprotQ16595
• Gene ID2395;
• Calculated MW 13kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.

• Western blot analysis of extracts of various cell lines, using FXN / Frataxin at 1:1000 dilution.
• Immunofluorescence analysis of 293T cells using FXN / Frataxin at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using FXN / Frataxin at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting