• Product NameHFM1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityMouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human HFM1 (NP_001017975.4).
• Alternative Names    HFM1; MER3; POF9; SEC63D1; Si-11; Si-11-6; helicase; probable ATP-dependent DNA helicase HFM1
• Accession No. Swiss-Prot#:A2PYH4
  NCBI Gene ID:164045
• UniprotA2PYH4
• Gene ID164045;
• Calculated MW 168kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9).

• Western blot analysis of extracts of various cell lines, using HFM1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting