• Product NameNR1H4 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityMouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human NR1H4 (NP_001193908.1).
• Alternative Names    NR1H4; BAR; FXR; HRR-1; HRR1; PFIC5; RIP14; bile acid receptor
• Accession No. Swiss-Prot#:Q96RI1
  NCBI Gene ID:9971
• UniprotQ96RI1
• Gene ID9971;
• Calculated MW 56kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described.

• Western blot analysis of extracts of various cell lines, using NR1H4 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting