• Product NameHAL Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human HAL (NP_002099.1).
• Alternative Names    HAL; HIS; HSTD; histidine ammonia-lyase
• Accession No. Swiss-Prot#:P42357
  NCBI Gene ID:3034
• UniprotP42357
• Gene ID3034;
• Calculated MW 73kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using HAL at 1:3000 dilution.
• Immunofluorescence analysis of C6 cells using HAL Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat kidney using HAL at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human lung cancer using HAL at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using HAL at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting