• Product Namebeta Tubulin antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 439 of Human TUBB
• Target Namebeta Tubulin
• Accession No. NCBI Gene ID: 203068
  NCBI mRNA#: NM_178014
  NCBI Protein#: NP_821133
• UniprotP07437
• Gene ID203068;
• Concentration 1.35 mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 50kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family. Dimer of alpha and beta chains.

• Sample (30 ug of whole cell lysate)
  A: 293T
  B: H1299
  10% SDS PAGE
  Primary antibody diluted at 1: 3000
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting