• Product NameIL25 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human IL25 (NP_073626.1).
• Alternative Names    IL25; IL17E; interleukin-25;IL-25
• Accession No. Swiss-Prot#:Q9H293
  NCBI Gene ID:64806
• UniprotQ9H293
• Gene ID64806;
• Calculated MW 24kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:200

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.

• Immunohistochemistry of paraffin-embedded rat lung using IL25 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human prostate using IL25 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse lung using IL25 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using IL25 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
• Immunofluorescence analysis of human lung cancer cells using IL25 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of rat bronchus cells using IL25 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of mouse lung cells using IL25 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting