• Product NameFLIP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationDEAE purified
• ApplicationsELISA WB ICC
• Species ReactivityMs
• SpecificityFLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa.
• Target NameFLIP
• Alternative Names    I-FLICE
• Accession No. Swiss-Prot:O15519
  Gene ID:8837
• UniprotO15519
• Gene ID8837;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 55 kd
  

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

• Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.
• Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting