• Product NameDR3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationDR3 Antibody is affinity chromatography purified via peptide column.
• ApplicationsELISA WB ICC
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor.
• Target NameDR3
• Alternative Names    Wsl-1 Apo-3
• Accession No. Swiss-Prot:Q93038
  Gene ID:8718
• UniprotQ93038
• Gene ID8718;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 59 kd
  

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-κB activation.

• Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
• Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 ug/mL.
• Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 5 μg/ml.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting