• Product NameHDAC5(Phospho-Ser498) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of HDAC5 only when phosphorylated at serine498.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 498 (T-Q-S(p)-S-P) derived from Human HDAC5/7.
• Target NameHDAC5
• ModificationPhospho
• Alternative Names    HD5
• Accession No. Swiss-Prot: Q9UQL6
  NCBI Protein: NP_001015053.1
• UniprotQ9UQL6
• Gene ID10014;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 124kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
   

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019.
McKinsey TA, et al. (2000) Nature. 408(6808): 106-111.
 

• Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody #11193.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Phospho-Ser498) Antibody #11193(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting