• Product Namec-Fos Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB IF
• Species ReactivityHu,Ms,Rt
• Immunogen DescriptionRecombinant protein of human c-Fos
• Target Namec-Fos
• Alternative Names    FOS; AP-1; C-FOS; p55; proto-oncogene c-Fos
• Accession No. Swiss-prot:P01100
  Gene ID:2353
• UniprotP01100
• Gene ID2353;
• SDS-PAGE MW 60kDa
• Storage Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.3.

• WB:1:500 - 1:2000

  IF:1:50 - 1:200

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2.  These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1.  As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.  In some cases, expression of the FOS gene has also been associated with apoptotic cell death. 

• Western blot analysis of extracts of various cell lines, using c-Fos antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
• Immunofluorescence analysis of NIH/3T3 cells using c-Fos antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of C6 cells using c-Fos antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting