• Product NameRecombinant Mouse Interleukin-36 gamma protein(Il36g) (Active)
• Brief DescriptionRecombinant Mouse Interleukin-36 gamma protein(Il36g) (Active)
• Host SpeciesE.coli
• Purification>98% as determined by SDS-PAGE and HPLC.
• Alternative Names    Interleukin-1 family member 9
• Accession No. Swiss-Prot#:Q8R460
• UniprotQ8R460
• Calculated MW 17.3 kDa
• Tag Info NO-tagged
• Target Sequence FGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

• Storage Buffer: 0.2 m filtered  20 mM Tris-HCl, pH 8.5, 150mM NaCl with Tween-20 ,lyophilized

  Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of ＞ 1.0 × 105 IU/mg.

  Relevance: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T cells and splenocytes. {ECO:0000250|UniProtKB:Q9NZH8, ECO:0000269|PubMed:21860022, ECO:0000269|PubMed:21965679}.

• Expression Region: 13-164aa

  Gene Names: Il36g,Il1f9

  Organism: Mus musculus (Mouse)

  Sequence Info : Full Length of Mature Protein

   Repeated freezing and thawing is not recommended. Store working aliquots at 4�?for up to one week.

 PubMed ID: 11991723; 15489334; 21860022; 21965679

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting