• Product NameZIPK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB ICC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids near the center of human ZIP kinase.
• Target NameZIPK
• Alternative Names    ZIPK
• Accession No. Swiss-Prot:O43293
  Gene ID:1613
• UniprotO43293
• Gene ID1613;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 40 kd
  

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

• Western blot analysis of ZIP kinase in HeLa (H) and Jurkat (J) whole cell lysates with ZIP kinase antibody at 1 ug/mL.
• Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 ug/mL.
• Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.
• Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.
• Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting