• Product NameCDT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CDT1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human CDT1
• Target NameCDT1
• Alternative Names    DUP; RIS2
• Accession No. Swiss-Prot:Q9H211
  NCBI Gene ID:81620
  NCBI Protein:NP_112190
• UniprotQ9H211
• Gene ID81620;
• Calculated MW 60 kDa
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 30-150

The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.

• Gel: 8%SDS-PAGE
  lysate: 40 ¦Ìg, Lane: Human fetal brain tissue lysate,
  Primary antibody: 46460£¨CDT1 Antibody) at dilution 1/400
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 20 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 46460(CDT1 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46460(CDT1 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting