• Product NameFoxO3a Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of mouse FoxO3a (NP_062714.1).
• Alternative Names    FOXO3;AF6q21;FKHRL1;FKHRL1P2;FOXO2;FOXO3A
• Accession No. GeneID:56484
  Swiss Prot:O43524
• UniprotO43524
• Gene ID2309;;
• Calculated MW 71kDa
• SDS-PAGE MW 90kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.64.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

• Immunohistochemistry of paraffin-embedded rat brain using FoxO3a antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded human liver damage using FoxO3a antibody at dilution of 1:100 .
• Immunofluorescence analysis of C6 cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using FoxO3a antibody at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting