• Product NameNFE2L1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human NFE2L1 (NP_003195.1).
• Alternative Names    NFE2L1;LCR-F1;NRF1;TCF11
• Accession No. GeneID:4779
  Swiss Prot:Q14494
• UniprotQ14494
• Gene ID4779;
• Calculated MW 81kDa/84kDa
• SDS-PAGE MW 64kDa
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 87% glycerol.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.40.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for 'nuclear respiratory factor 1' which has an official symbol of NRF1.

• Western blot analysis of extracts of various cell lines, using NFE2L1 antibody at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using NFE2L1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human lung cancer using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded human colon using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded human placenta using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded human gastric cancer using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded human uterus using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded mouse brain using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded rat spleen using NFE2L1 antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded rat heart using NFE2L1 antibody at dilution of 1:100 .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting