• Product NameCIDE-A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityIt has no cross activity to CIDE-B.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids 200 to 217 of human CIDE-A.
• Target NameCIDE-A
• Accession No. Swiss-Prot:O60543
  Gene ID:1149
• UniprotO60543
• Gene ID1149;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 23 kd
  

Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.

• Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.
• Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting