• Product NameMAPK4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MAPK4 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human MAPK4
• Target NameMAPK4
• Alternative Names    ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
• Accession No. Swiss-Prot:P31152
  NCBI Gene ID:5596
  NCBI Protein:NP_002738
• UniprotP31152
• Gene ID5596;
• Calculated MW 66 kDa
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 30-150

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants.

• Gel: 8%SDS-PAGE
  lysate: 40 ¦Ìg, Lane 1-4: 293T cell£¬Human heart tissue£¬Raji and LNCAP cell lysates,
  Primary antibody: 46605£¨MAPK4 Antibody) at dilution 1/550
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46605(MAPK4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46605(MAPK4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting