• Product NameNCAPH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NCAPH protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic protein corresponding to residues near the C terminal of human NCAPH
• Target NameNCAPH
• Alternative Names    BRRN1; CAP-H
• Accession No. Swiss-Prot:Q15003
  NCBI Gene ID:23397
  NCBI Protein:BC024211
• UniprotQ15003
• Gene ID23397;
• Calculated MW 83 kDa
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 30-150

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

• Gel: 6%SDS-PAGE
  £¬ Lysate: 40 ¦Ìg£¬ Lane 1-2:K562 and Jurkat cell lysates£¬
  Primary antibody: 46621(NCAPH Antibody) at dilution 1/450£¬
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution£¬
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 46621(NCAPH Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 46621(NCAPH Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting