• Product NameNREP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NREP protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to internal residues of human NREP
• Target NameNREP
• Alternative Names    P311; PTZ17; SEZ17; D4S114; C5orf13; PRO1873
• Accession No. Swiss-Prot:Q16612
  NCBI Gene ID:9315
  NCBI Protein:NP_001135947
• UniprotQ16612
• Gene ID9315;
• Calculated MW 8 kDa
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1: 20-100

NREP may have roles in neural function. Ectopic expression augments motility of gliomas. Promotes also axonal regeneration. May also have functions in cellular differentiation. Induces differentiation of fibroblast into myofibroblast and myofibroblast ameboid migration. Increases retinoic-acid regulation of lipid-droplet biogenesis. Down-regulates the expression of TGFB1 and TGFB2 but not of TGFB3. May play a role in the regulation of alveolar generation.

• Gel: 12%SDS-PAGE
  lysate: 40 ¦Ìg, Lane 1-2: A172 cell and Rat heart tissue lysates,
  Primary antibody: 46630£¨NREP Antibody) at dilution 1/200
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46630(NREP Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46630(NREP Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting