• Product NamePRPF8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PRPF8 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human PRPF8
• Target NamePRPF8
• Alternative Names    PRP8; RP13; HPRP8; PRPC8; SNRNP220
• Accession No. Swiss-Prot:Q6P2Q9
  NCBI Gene ID:10594
  NCBI Protein:NP_006436
• UniprotQ6P2Q9
• Gene ID10594;
• Calculated MW 274 kDa
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 20-100

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

• Gel: 6%SDS-PAGE
  lysate: 40 ¦Ìg, Lane 1-5: 293T£¬K562£¬Raji£¬Hela and NIH/3T3 cell lysates,
  Primary antibody: 46649£¨PRPF8 Antibody) at dilution 1/600
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 50 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46649(PRPF8 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46649(PRPF8 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting