• Product NameIL-1RAcP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThis polyclonal antibody has no cross activity to other members in the IL-1 receptor family.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids 525 to 540 of human IL-1RAcP, which is identical to those of mouse and rat origins.
• Target NameIL-1RAcP
• Alternative Names    IL-1RAcP
• Accession No. Swiss-Prot:Q9NPH3
  Gene ID:3556
• UniprotQ9NPH3
• Gene ID3556;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 66 kd
  

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-κB inducing kinae (NIK) and of NF-κB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

• Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody (C2) at 1:1000 dilution.
• Immunofluorescence of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.
• Immunocystochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 μg/ml.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting